User:Morgan L. Paull/Notebook/C.Dog V1.0 - T7 Promoter/2011/07/24
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Picking colonies from Friday's full library transformation
All look good, either red or green fluorescent under the light as appropriate, except for the following:
I think that the way to test this moving forward to to inoculate G18.1 and R8.2 in LB + Kan + IPTG to see if they fluoresce properly under induced conditions.
Other than these two, the cultures all look great. The plate was not overgrown but had plenty of colonies in all cases. I can't find the plate of direct-from-transformation glycerol stocks/overnight cultures that Andy finished for me on Saturday, so I assume it is done and frozen, forming another backup for me.
Ligations from Friday