User:Morgan L. Paull/Notebook/C.Dog V1.0 - T7 Promoter/2011/07/19

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Checking Sequences for another round of ligations

  • R24L6.1 and R24L6.2 are both good (take 6.1, it's a perfect alignment with RFP, showed up in the library search).
  • R23L6.1 and R23L6.2 are both good (take 6.1, it's also a perfect alignment with RFP, showed up in the library search).
  • R18L5.1 and R18L5.2 don't seem to have the inserts, only RFP
  • R17L6.1 and R17L6.2 are both good, both perfect alignments to all parts
  • R17L5.1 and R17L5.2 don't have inserts, only RFP
  • R16L6.1 and R16L6.2 are both good (take 6.2, it has a perfect alignment to RFP, showed up in the library search).
  • R16L5.1 and R16L5.2 both don't have inserts, only RFP

Bottom line: All of L5 ligations are re-ligated backbone and do not have inserts. Do not repeat ligations with L5 backbone.

Have finished and completed:

  • R24L6.1
  • R23L6.1
  • R17L6.1
  • R16L6.2


Need to re-do: R18, R22 (already re-tried R22, but should re-retry it again for safety, low efficiency on the transformations).