User:Morgan L. Paull/Notebook/C.Dog V1.0 - T7 Promoter/2011/06/21
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Nanodroped GFP and RFP backbone plasmids
Digesting Backbones with BsaI
Began incubating each in 37°C water bath at 11:45 am, removed at 2:45pm, ran on .7% agarose gel, cut out the lowest band (digested backbone). Performed gel purification protocol, skipping isopropanol step at Colin's advice.
Received in mail:
Centrifuged tubes at 15,000 rpm for 10 mins, resuspended with (nmol*10) μL of DH2O:
In PCR Tubes
In PCR machine
Removed the completed reaction tubes at the end of the day, and put them in my box in the -20°C freezer.