User:Monika Gasiorek/Notebook/CHEM-571 2014F/2015/03/25
From OpenWetWare
< User:Monika Gasiorek | Notebook | CHEM-571 2014F | 2015 | 03
Jump to navigationJump to search Project name
|
 Main project page Previous entry Next entry
|
March 25th, 2015Proteinase K Bradford250 μL samples of protease reaction solutions in Tris/CaCl2 buffer at the experimental concentrations (1 nM, 10 nM, 100 nM, and 1 μM) were combined with 200 mL of 1:4 (Bradford:Buffer) Bradford reagent and 550 mL of additional Tris/CaCl2 buffer. UV-Vis spectra were obtained from 400-800 nm and corrected for any absorbance due to protease in the reaction solutions.
| |
