User:Monika Gasiorek/Notebook/CHEM-571 2014F/2015/03/25

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March 25th, 2015

Proteinase K Bradford

250 μL samples of protease reaction solutions in Tris/CaCl2 buffer at the experimental concentrations (1 nM, 10 nM, 100 nM, and 1 μM) were combined with 200 mL of 1:4 (Bradford:Buffer) Bradford reagent and 550 mL of additional Tris/CaCl2 buffer. UV-Vis spectra were obtained from 400-800 nm and corrected for any absorbance due to protease in the reaction solutions.

Proteinase K Bradford 2 1 uM.png

Proteinase K Bradford 2 100 nM.png

Proteinase K Bradford 2 10 nM.png

Proteinase K Bradford 2 1 nM.png