User:Monika Gasiorek/Notebook/CHEM-571 2014F/2015/02/11
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February 11th, 2015
In situ Kinetics
Ocean Optics will be run at 37°C, scanning every 5 minutes for 6 hours.
The incorrect stock solution was used to make the protease solution. The concentration being tested is not 1 nM. It was calculated to be 1 µM for protease. This reaction will be re-run tomorrow with the proper enzyme concentration.
Bradford Analysis of Proteinase K Reaction Samples
250 μL of each protein sample at every time point (collected yesterday) was combined with 200 μL of Bradford reagent (1:4 Bradford:Tris/NaCl), and 1.550 mL of Tris/NaCl buffer and run from 400 - 800 nm on the UV/Vis spectrophotometer. The following are the results with the blank subtracted out and zeroed at a wavelength of 535 nm.