User:Monika Gasiorek/Notebook/CHEM-571 2014F/2015/01/27

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

January 27th, 2015

Stock Solution Preparation

1. 100 mL of 2.5 mM HAuCl4 solution was made from 0.08539 g of HAuCl4 (MW 339.785 g/mol) in 100 mL of distilled H2O

2. 100 mL of 20 μM lysozyme solution was made from 0.02861 g of lysozyme (MW 14300 Da) in 100 mL of distilled H2O

AuNP Fiber Preparation

50 - 5 mL tubes of 45:1 [Au]:[Lysozme] AuNP solution were prepared with final concentrations of 0.25 mM and 5.56 μM of Au and lysozyme, respectively. Each tubed contained the following:

  • 0.5 mL of 2.5 mM HAuCl4
  • 1.39 mL of 20 μM lysozyme
  • 3.11 mL of distilled H2O

The AuNP solutions were placed in the oven at 80°C for four hours.

Protease and Buffer Preparation

1. 8 samples of approximately 4 mg of pepsin were prepared.

  • 1. 0.00530 g
  • 2. 0.00398 g
  • 3. 0.00391 g
  • 4. 0.00470 g
  • 5. 0.00419 g
  • 6. 0.00465 g
  • 7. 0.00780 g
  • 8. 0.00584 g


2. 1000 mL of 5 mM Tris/0.2 M NaCl buffer was prepared from 11.68960 g of NaCl and 0.78922 g of Tris in approx. 1000 mL of distilled H2O. 0.5 N HCl was added dropwise to a pH of 8.067.