User:Monika Gasiorek/Notebook/CHEM-571 2014F/2015/01/27

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January 27th, 2015 Stock Solution Preparation 1. 100 mL of 2.5 mM HAuCl4 solution was made from 0.08539 g of HAuCl4 (MW 339.785 g/mol) in 100 mL of distilled H2O 2. 100 mL of 20 μM lysozyme solution was made from 0.02861 g of lysozyme (MW 14300 Da) in 100 mL of distilled H2O AuNP Fiber Preparation 50 - 5 mL tubes of 45:1 [Au]:[Lysozme] AuNP solution were prepared with final concentrations of 0.25 mM and 5.56 μM of Au and lysozyme, respectively. Each tubed contained the following: 0.5 mL of 2.5 mM HAuCl4 1.39 mL of 20 μM lysozyme 3.11 mL of distilled H2O The AuNP solutions were placed in the oven at 80°C for four hours. Protease and Buffer Preparation 1. 8 samples of approximately 4 mg of pepsin were prepared. 1. 0.00530 g 2. 0.00398 g 3. 0.00391 g 4. 0.00470 g 5. 0.00419 g 6. 0.00465 g 7. 0.00780 g 8. 0.00584 g 2. 1000 mL of 5 mM Tris/0.2 M NaCl buffer was prepared from 11.68960 g of NaCl and 0.78922 g of Tris in approx. 1000 mL of distilled H2O. 0.5 N HCl was added dropwise to a pH of 8.067.