User:Monika Gasiorek/Notebook/CHEM-571 2014F/2015/01/27
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January 27th, 2015
Stock Solution Preparation
1. 100 mL of 2.5 mM HAuCl4 solution was made from 0.08539 g of HAuCl4 (MW 339.785 g/mol) in 100 mL of distilled H2O
2. 100 mL of 20 μM lysozyme solution was made from 0.02861 g of lysozyme (MW 14300 Da) in 100 mL of distilled H2O
AuNP Fiber Preparation
50 - 5 mL tubes of 45:1 [Au]:[Lysozme] AuNP solution were prepared with final concentrations of 0.25 mM and 5.56 μM of Au and lysozyme, respectively. Each tubed contained the following:
The AuNP solutions were placed in the oven at 80°C for four hours.
Protease and Buffer Preparation
1. 8 samples of approximately 4 mg of pepsin were prepared.