User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/10/29

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October 29, 2014

Primary Experiment #2 - Work-up Take 2

30:1 colloid solution vs CaCl2 using MWCO 3500

Well 1 Well 2 Well 3 Well 4 Well 5
30:1 colloid 30:1 colloid 30:1 colloid 30:1 colloid 30:1 colloid
50 mM CaCl2 5 mM CaCl2 0.5 mM CaCl2 50 μM CaCl2 5 μM CaCl2

Necessary Analyses:

Bradford

  • 5 colloid samples, 30:1 colloid reference, and Bradford blank
  • 100 μL sample, 200 μL 1:4 Bradford, 700 μL 50 mM Tris/50 mM NaCl

Bradford Colloid CaCl2 3500MW 10 29 Chart.png

Fluorescence & UV-VIS not necessary per NOTE on 10/21

CaCl2 ISE

  • 10 samples
Concentration Colloid Solution Potential (mV) Soak Solution Potential (mV)
50 mM 82.2 77.2
5 mM 58.8 56.8
0.5 mM 46.2 40.5
50 µM 41.6 36.0
5 µM 35.7 32.9

Primary Experiment #4 - Set-up

0.6 g/L Lysozyme vs HCl using MWCO 20000

Well 1 Well 2 Well 3 Well 4 Well 5
0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozome
5 mM HCl 500 μM HCl 250 μM HCl 100 μM HCl 75 μM HCl