User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/10/28

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

October 28, 2014

Primary Experiment #1 - KI Analysis

Because the titrations done last week demonstrated inconsistent results, the KI concentrations of each of the ten dialysis samples were re-analyzed using the self-made Iodide Ion Selective Electrode. The results are as follows:

Sample Potential (mV) ln[KI] [KI] (mM) Sample Potential (mV) ln[KI] [KI] (mM)
L+50 mM KI -253.7 3.014259251 20.37399334 50 mM KI N/A N/A N/A
L+25 mM KI -237.7 2.324990307 10.22658096 25 mM KI -240.1 2.428380649 11.34050295
L+10 mM KI -215.8 1.38155344 3.981081197 10 mM KI -210.8 1.166156895 3.209633945
L+5 mM KI -199.9 0.696592427 2.006902376 5 mM KI -196.2 0.537198983 1.711207024
L+2 mM KI -177.4 -0.272692026 0.761327221 2 mM KI -173.7 -0.432085469 0.649153892


NOTE: No more 50 mM KI sample remained to test with I- ISE due to an error that occurred during the titration.

Primary Experiment #3 - Work-up

0.6 g/L Lysozyme vs CaCl2 using MWCO 3500

Well 1 Well 2 Well 3 Well 4 Well 5
0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozome
50 mM CaCl2 5 mM CaCl2 0.5 mM CaCl2 50 μM CaCl2 5 μM CaCl2

Necessary Analyses:

Bradford

  • 5 lysozyme samples, 0.6 g/L lysozyme reference, and Bradford blank
  • 50 μL sample, 200 μL 1:4 Bradford, 750 μL 50 mM Tris/50 mM NaCl

Lysozyme CaCl2 Bradford 3500MW 10 28 Chart.png

Fluorescence

  • 5 lysozyme samples, 0.6 g/L lysozyme reference
  • 10 μL of sample in 990 μL of DI water

10 28 Lysozyme CaCl2 Fluorescence Chart.png

UV-Vis

  • 5 lysozyme samples, 0.6 g/L lysozyme reference
  • 10 μL of sample in 990 μL of DI water

Lysozyme CaCl2 UV 10 29 Chart.png

NOTE: The 5 µM sample was re-run because the wavelength range was not correct the first time the sample(s) was/were run. A lysozyme stock reference sample and Bradford/Tris/NaCl blank were re-run for comparison as well.

CaCl2 ISE

Concentration Lysozyme Solution Potential (mV) Soak Solution Potential (mV)
50 mM 88.6 87.8
5 mM 61.3 63.5
0.5 mM 30.0 39.0
50 µM 10.5 16.3
5 µM 8.9 26.0

Primary Experiment #2 - Set-up Take 2

30:1 colloid solution vs CaCl2 using MWCO 3500

NOTE: Because the results of the Bradford analysis of this dialysis (take 1) were ridiculous, for lack of a better word, this dialysis was re-run and re-analyzed.

Well 1 Well 2 Well 3 Well 4 Well 5
30:1 colloid 30:1 colloid 30:1 colloid 30:1 colloid 30:1 colloid
50 mM CaCl2 5 mM CaCl2 0.5 mM CaCl2 50 μM CaCl2 5 μM CaCl2