User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/10/28

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October 28, 2014 Primary Experiment #1 - KI Analysis Because the titrations done last week demonstrated inconsistent results, the KI concentrations of each of the ten dialysis samples were re-analyzed using the self-made Iodide Ion Selective Electrode. The results are as follows: Sample Potential (mV) ln[KI] [KI] (mM) Sample Potential (mV) ln[KI] [KI] (mM) L+50 mM KI -253.7 3.014259251 20.37399334 50 mM KI N/A N/A N/A L+25 mM KI -237.7 2.324990307 10.22658096 25 mM KI -240.1 2.428380649 11.34050295 L+10 mM KI -215.8 1.38155344 3.981081197 10 mM KI -210.8 1.166156895 3.209633945 L+5 mM KI -199.9 0.696592427 2.006902376 5 mM KI -196.2 0.537198983 1.711207024 L+2 mM KI -177.4 -0.272692026 0.761327221 2 mM KI -173.7 -0.432085469 0.649153892 NOTE: No more 50 mM KI sample remained to test with I- ISE due to an error that occurred during the titration. Primary Experiment #3 - Work-up 0.6 g/L Lysozyme vs CaCl2 using MWCO 3500 Well 1 Well 2 Well 3 Well 4 Well 5 0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozyme 0.6 g/L lysozome 50 mM CaCl2 5 mM CaCl2 0.5 mM CaCl2 50 μM CaCl2 5 μM CaCl2 Necessary Analyses: Bradford 5 lysozyme samples, 0.6 g/L lysozyme reference, and Bradford blank 50 μL sample, 200 μL 1:4 Bradford, 750 μL 50 mM Tris/50 mM NaCl Fluorescence 5 lysozyme samples, 0.6 g/L lysozyme reference 10 μL of sample in 990 μL of DI water UV-Vis 5 lysozyme samples, 0.6 g/L lysozyme reference 10 μL of sample in 990 μL of DI water NOTE: The 5 µM sample was re-run because the wavelength range was not correct the first time the sample(s) was/were run. A lysozyme stock reference sample and Bradford/Tris/NaCl blank were re-run for comparison as well. CaCl2 ISE Concentration Lysozyme Solution Potential (mV) Soak Solution Potential (mV) 50 mM 88.6 87.8 5 mM 61.3 63.5 0.5 mM 30.0 39.0 50 µM 10.5 16.3 5 µM 8.9 26.0 Primary Experiment #2 - Set-up Take 2 30:1 colloid solution vs CaCl2 using MWCO 3500 NOTE: Because the results of the Bradford analysis of this dialysis (take 1) were ridiculous, for lack of a better word, this dialysis was re-run and re-analyzed. Well 1 Well 2 Well 3 Well 4 Well 5 30:1 colloid 30:1 colloid 30:1 colloid 30:1 colloid 30:1 colloid 50 mM CaCl2 5 mM CaCl2 0.5 mM CaCl2 50 μM CaCl2 5 μM CaCl2