User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/10/15
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October 15, 2014
SDS-PAGE: Running of the Gels
The four samples prepared yesterday were heated to 40C on a heating block. Afterwards, they were transferred into a premade gel along with a premade marker/ladder. The gel was then run under the same conditions as done earlier in the semester. (See notes from September 23, 2014)
The following table depicts the placement of our samples in the 12-well gel:
And the rest is history...
Primary Experiment Dialysis #2 Work-up
30:1 colloid solution vs CaCl2 using MWCO 3500
NOTE: There was a bit of a struggle getting a reliable spectrum for the blank. Was run 3 times in order to average/verify. We also made the executive decision to run samples of 50 μL from now on (diluted with 200 μL Bradford and 750 μL Tris/NaCl) in order to increase the precision/accuracy of the results.
Primary Experiment Dialysis #3 Set-Up
0.6 g/L Lysozyme vs CaCl2 using MWCO 3500