User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/10/07
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October 7, 2014
Continuation of 3500 MWCO Dialysis Analysis
Bradford analysis of all protein-containing samples was completed last week.
Fluorescence analysis was done on all protein containing solutions of this dialysis, including:
Emission spectra were obtained on a range of 300 to 550 nm with an excitation wavelength of 280 nm. The cuvette was cleaned with 1 N HCl, HPLC water, and methanol between runs to prevent protein residue build-up. Each sample was diluted 100 fold (10 μL in 990 μL).
NOTE:The same procedure was followed first for the listed soak solution samples, only realizing after obtaining each spectra that fluorescence data was only necessary for protein-containing samples.
The fluorimetry results will be posted as soon as possible.
UV Absorption spectra were obtained for each of the crude, protein-containing samples mentioned above on a 200-800 nm range. The cuvettes were cleaned in the same manner as with fluorescence.
Ca2+ concentration in Ca2+ containing samples (wells 4 and 5) will be calculated using our pre-determined calibration curve given the following ISE hardwater probe conductivities(?):
pH measurements were obtained for all samples containing H+ ions (well 2) with the following results:
20 mL of 30:1 [Au]:[Lysozyme] of new colloid solution was made.