User:Monika Gasiorek/Notebook/CHEM-571 2014F/2014/09/02
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September 2, 2014
Titrant Stock Solution Preparation and Standardization
500 mL of approximately 0.150 M NaOH solution was prepared from 2.99978 g of solid NaOH and 500 mL of H2O.
The titrant was standardized in order to determine the actual concentration.
NOTE:The first trial did not result in a color change. The second trial required approx. 25 mL of NaOH for a color change to be observed. The error here could possibly be due to the failure to completely dissolve all of the solid NaOH crystals in 500 mL of water. The stock bottle was shaken properly before the 3rd and 4th trials, resulting in reproducible results.
The calculated average for the NaOH titrant stock solution was 0.1575 M.
The data from the titrant standardization is included below.
Hydrophobic Amino Acid Titration: Valine
A solution of valine was titrated with the 0.1575 M NaOH titrant in order to produce a characteristic titration curve for valine.
A pH probe was calibrated and used to track the change in pH as additional amounts of 0.1575 M NaOH were added over time.