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  • Staining WGA injected mice

3 Brain slices (30um) of 3 CRH-CreXTdTom-Flox mice injected with AAV-Dio-WGA-GFP in the PVN were stained with primary (Rabbit) anti GFP antibodies.

500x4=2000 ul final volunme Rabit GFP AB 1:500 ⇒ 4ul Donkey Serum 3% ⇒ 60ul TritonX 0.3% ⇒ 6ul 100% => 60ul 10% PBS 1876ul