User:Michaela Harper/Notebook/Biological Chemistry Lab/2012/02/14
|Effect of Tris Buffer on AuNP||Main project page|
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To continue the experiment performed on 02/01/12 to determine the effects of various concentrations of tris buffer on AuNP absorbance.
The following graph shows a zoom-in at 550nm on the spectra obtained by varying the concentration of Tris buffer added to the AuNPs.
The addition of more concentrated tris buffer appeared to have a small effect on the AuNPs overall. However, the nanoparticles had a decreased absorbance with the addition of more buffer in solution. Also, all additions of tris buffer resulted in a shift of the apex of the peak towards shorter wavelengths (blue-shift).