User:Michaela Harper/Notebook/Biological Chemistry Lab/2012/02/08

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To make a solution of gold nanoparticles (purple solution) using the ratio of 70.


  1. Combine 2 mL of 3.66 mM HAuCl4 and 6.04 mL of 0.0173 mM BSA in a test tube.
  2. Dilute to 20 mL with water.
  3. Place in oven at 74°C.
  4. Leave solutions in oven for 3.5 hr.
  5. Continue to take UV-vis data of 0.9 mL 70 Au/BSA ratio + 0.1 mL 50 mM tris buffer solution over time.
  6. Continue to take UV-vis data of 1 mL 70 Au/BSA ratio solution over time.


The following graph shows the response of the gold nanoparticle solution to time, expressed between week 1 when the solution was made, and week 2. The response increased over this time.

Week 2.png


UV-vis data for AuNP + 50 mM Tris (pH 10.1) solution (made on 02/01/12) could not be taken, as the AuNP precipitated out of solution.

Preciptated AuNP.png