User:Michaela Harper/Notebook/Biological Chemistry Lab/2012/02/01

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Objective

Determine the effects of tris buffer (pH 10.1) using UV-Vis on purple nanoparticle solution and protein fiber solution as a function of concentration and time.

Description

  1. Take UV-Vis data of 70 and 166 Au/BSA solutions prepared on 01/31/12
  2. Take UV-Vis data of 0.9 mL of each AuNP solution + 0.1 mL of the following dilutions of tris (pH 10.1)
    • 50 mM
    • 5.0 mM
    • 0.50 mM
    • 0.050 mM
  3. Continue to take UV-vis data of 0.9 mL 70 Au/BSA ratio + 0.1 mL 50 mM tris buffer solution over time (first dilution).

Data

The following graphs represent the data obtained in the analysis of the effect of time on the gold nanoparticles synthesized using the ratio of 70 for BSA/Au.


70-response to time.png

Time-550 nm zoom.png

Time v. absorbance.png


The following graphs represent the data obtained in the analysis of the effect of ionic strength (concentration of tris buffer) on the gold nanoparticles synthesized using the ratio of 70 for BSA/Au.

Ionic strength-70.png

Ionic strength 70-zoom.png

Concentration v. absorbance.png


The following graphs represent the data obtained in the analysis of the effect of ionic strength (concentration of tris buffer) on the gold nanoparticles synthesized using the ratio of 166 for BSA/Au.

Ionic strength 166.png

Ionic strength 166 zoom.png

Concentration v. absorbance- 166.png

Notes

The response of absorbance at 550 nm for the 70 ratio solution seems to increase with time, but decrease with increasing concentration of tris buffer added.

The response for the 166 ratio seems relatively unaffected by the addition of buffer.