User:Michaela Harper/Notebook/Biological Chemistry Lab/2011/11/15

From OpenWetWare
Jump to: navigation, search
BDLlogo notext lr.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


Objective

To attempt to stop BSA refolding and to determine in what situations BSA/AuNP fibers are stable.

Description

  1. Make two solutions of AuNP.
    • Solution 1 (aqueous AuNP):
      1. Combine 0.516 mL of 30 μM BSA and 0.350 mL of 4.17 mM HAuCl4 in a test tube.
      2. Place in 80°C oven until solution turns purple.
    • Solution 2 (AuNP/BSA fibers):
      1. Combine 0.516 mL of 30 μM BSA and 0.700 mL of 4.17 mM HAuCl4 in a test tube.
      2. Place in 80°C oven for 2 hr.
  2. Remove solution 1 from oven.
  3. Add 100 μL of 5.02 mM tris (pH 10.1) to 1 mL of solution 1.
  4. Take UV vis data.
  5. Repeat steps 3 and 4 two more times.

Data

Solution 1 began to turn purple after 1.5 hr in the oven. It was removed at this time to test nanoparticle stability using a Tris buffer. Small purple fibers formed in solution 2.


UV-Vis data from the Tris buffer experiment is as follows:

Absorbance vs wavelength 11-15-11.jpg


Absorbance vs wavelength magnified 11-15-11.jpg

Notes