User:Michaela Harper/Notebook/Biological Chemistry Lab/2011/10/05
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To continue the experiment in determining the sensitivity of the fluorimeter and to purify DNA made by cells that were cultured on 09/27/11.
1. Fluorimeter Sensitivity II
The DNA purification protocol was adapted from the Wizard Plus SV Minpreps DNA Purification System vacuum protocol found here:
Fluorimeter Sensitivity II
This graph shows the integral of fluorescence intensity vs. the concentration of Ru(bpy)(phen). The graph begins to level off, indicating that the Ru(bpy)(phen) is emitting too much light for the fluorimeter.
Calculated concentrations of MBP dilutions:
40 μM: 2001.315233
60 μM: 2237.400074
80 μM: 2294.581483
100 μM: 2286.935912