User:Michaela Harper/Notebook/Biological Chemistry Lab/2011/09/28
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To repeat gold nanoparticle synthesis and to continue protein expression started on 09/27/11.
1. Nanoparticle Synthesis
2. Protein Expression II
The absorbance values for the AuNP solution were corrected by subtracting the absorbance values for a blank acetate buffer. The corrected absorbance values at 550 nm were graphed against time. The graph shows a linear relationship after 60 min, indicating that AuNPs were formed in solution.
This graphs shows the corrected absorbance against wavelength. Absorbance data collected at 150 min from 200 nm-300 nm was ignored, because of outside interference with the UV-vis instrument.
A magnified version of this graph shows that absorbance values at around 550 nm increased over time, indicating the creation of AuNPs.