Creating a New Fluorescence Calibration Curve
- A calibration curve relating the fluorescence of Rhodamine B to its concentration will be created from data gathered today.
- The fluorescence responses of Rhodamine B were anomalous, with certain larger concentrations fluorescing at a lower intensity than certain lower concentrations. It was determined that this may be the result of dimerization at lower concentrations. It is necessary to perform UV-Vis on the solutions prior to fluorescence analysis in order to determine if there may be some dimerization causing the strange results. Unfortunately, the UV-Vis was being used and attempts to set up a new UV-Vis spectrophotometer fell short. As soon as UV-vis is available, we will test our standard solutions in this way.
- Fluorescence data was not removed from the fluorescence computer prior to Spring Break so worked-up data will not be available until at least next week.