User:Michael S. Bible/Notebook/CHEM-671/690 Lab Notebook/2016/02/03

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Objectives

  • Begin synthesis of new set of fiber samples
  • Use fluorimetry to analyze supernatants of fiber samples produced on January 27th
  • Use fluorimetry to analyze supernatants of fiber samples produced on February 2nd
  • Use fluorimetry to analyze initial samples prior to being placed in oven.

Protocol

  • Fiber samples were spun down for 2 minutes at 13000 RPM.
  • 200 μL of supernatant was then placed in the fluorescence spectrometer and the emission spectrum from 540 to 700 nm was taken using an excitation wavelength of 520 nm.