User:Michael S. Bible/Notebook/CHEM-671/690 Lab Notebook/2015/11/04
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Bradford Analysis of Protease Degradation
The general procedure today was taken from Dr. Hartings's notebook.
All samples will be incubated in eppendorf tubes, which will be placed in the 37°C water bath on the shaker. Samples should all contain the same concentration of materials.
You should make samples for measurements at 10 minutes, 15 minutes, 30 minutes, 45 minutes, 1 hr, 1.5 hr, 2 hr. (Note, this is for the first time you do this. As you get better, I will expect you to get more measurements done).
The spectrum for the overall blank with no protease or fiber sample was subtracted from all other spectra (blank and sample spectra). The spectra were further corrected by subtracting the absorbance at the isosbestic point.