User:Michael S. Bible/Notebook/CHEM-671/690 Lab Notebook/2015/10/27
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Fluorescence Analysis of Protease Degradation
We are using the Pierce quantitative fluorometric peptide assay. (product link here)
The general procedure for today was taken from Dr. Hartings's Notebook
All samples will be incubated in eppendorf tubes, which will be placed in the 37 °C water bath on the shaker. Samples should all contain the same concentration of materials.
You should make samples for measurements at 10 minutes, 15 minutes, 30 minutes, 45 minutes, 1 hr, 1.5 hr, 2 hr.
Fluorescence data was analyzed by subtracting each blank spectrum from its respective sample spectrum. The spectra were then integrated from 410 nm to 650 nm on Excel by using the midpoint method. The integration values were then converted to concentration values using the calibration curve created on 9/29.