User:Michael S. Bible/Notebook/CHEM-671/690 Lab Notebook/2015/09/15
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Nanoparticle fiber synthesis begun on September 2nd was unsuccessful. Only nanoparticle solutions were created, so today we will try to synthesize new samples of Au-NP Fibers.
Stock solutions of HAuCl4 and Lysozyme were prepared by Dr. Hartings using the procedure found here.
5mL and 1 mL samples were created using the following procedure:
Note: The final concentration of gold should be 0.25 mM and the final concentration of lysozyme should be 5.556 μM. This gives a Au:lysozyme ratio of 45, which should yield fibers.
The solutions were covered by aluminum foil and placed in the oven at 80°C for 4 hours.
The graph above was created using the abosrbance values at λ = 280 nm in the UV-Vis spectra of lysozyme collected by the entire class. The extinction coefficient for lysozyme at λ = 280 nm, calculated from this data set, is ε = 3.32·104M-1cm-1.