User:Michael S. Bible/Notebook/571/2014/11/05

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Objective

  • Analyze Colloid Dialysis began yesterday
  • Perform kinetics experiment described below
  • Verify concentrations of CaCl2 solutions used via ISE

Description

  • Enzyme Kinetics:
    • Use kinetics mode on UV-VIS
    • Prepare 10mL 400 unit/mL of lysozyme
      • use cold buffer
      • you may want to use your 0.6 or 0.12 g/L solution & dilute)
      • follow procedure of protocol here.
    • Repeat protocol using 4 solutions between 200 & 400 units/mL
      • You only need 1 mL of each of these
      • Prepare just before addition to keep solution cold
  • Set up new dialysis (if time)

Data

Actual Concentrations of Stock CaCl2 Solutions

Ca2+ Stock Solution Concentrations

Believed CaCl2 Concentration (mM) mV Measurement (mV) Actual CaCl2 Concentration (mM)
45 84.2 37.8
35 83.1 34.24
25 79.7 25.16
15 74.9 16.29
5 63.3 5.7


Ca2+ ISE for Colloid dialzyed against CaCl2

Original CaCl2 Concentration (mM) mV Measurement (mV) Final CaCl2 Concentration (mM)
37.8 77.8 21.19 (bad data point)
34.24 74.8 16.14
25.16 70.6 11.04
16.29 65.5 6.95
5.7 52.6 2.16

Notes

The colloids crashed out in the 45, 35, 25, and 15 mM CaCl2 dialysis wells from yesterday.