User:Michael S. Bible/Notebook/571/2014/10/29

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Objective

  • Create new CaCl2 solutions to dialyze against.
  • Collect data from dialysis started yesterday.
  • Start a new dialysis of Lysozyme against CaCl2.


Data

Ca2+ ISE for Lysozyme dialzyed against CaCl2

Original CaCl2 Concentration (mM) mV Measurement (mV) Final CaCl2 Concentration (mM)
50 78.5 22.57
5 52.8 2.20072
0.5 20.8 0.1213
0.05 1.8 0.0217
0.005 -0.3 0.0179 (This is a bad data point)

Notes

The Bradford analysis of this sample turned out poorly again and did not yield workable results.

New CaCl2 solutions have the following concentrations:

  • 6.83 mM
  • 1.87 mM
  • 0.68 mM
  • 0.020 mM
  • 0.00058 mM