User:Michael S. Bible/Notebook/571/2014/10/15

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Fluorescence Analysis of CaCl2-Dialyzed Lysozyme

  • The Fluorescence Spectrometer was used to analyze the dialyzed Lysozyme.
  • The instrument was set to an excitation wavelength of λ = 280 nm.
  • Emission spectra were collected in the range of λ = 300-550 nm.
  • Spectra data were integrated using Riemann sums of base 0.5 nm.

Fluorescence of CaCl2-Dialyzed Protein Data

Fluorescence Responses of Calcium binded proteins.png

In the image above, there is a clear and highly linear relationship between the peak integration and log[CaCl2] between 50 μM and 5 mM concentrations of CaCl2. Additional tests should be done to determine if this is indeed the case and to see if the trend extends further in either direction.

Fluorescence Analysis of HCl-Dialyzed Lysozyme

  • The Fluorescence Spectrometer was used to analyze the dialyzed Lysozyme and the solutions against which Lysozyme was dialyzed.
  • The instrument was set to an excitation wavelength of λ = 280 nm.
  • Emission spectra were collected in the range of λ = 300-550 nm.
  • Spectra data were integrated using Riemann sums of base 0.5 nm.

Fluorescence of HCl-Dialyzed Protein Data

Concentration of HCl versus peak integration of post-dialysis HCl soaks.png

In the image above, there is no clear relationship between the concentration of the HCl soak initially and its post-dialysis peak integration.

Concentration of HCl versus peak integration of post-dialysis protein solutions.png

There appears to be a highly linear relationship between the peak integration values at HCl concentration of 250 μM, 125 μM, and 30 μM. More tests are necessary to see if this is indeed the case.

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


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