User:Michael Corrado/Notebook/Experimental Chemistry Fall 2016/2017/09/05

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Objective

  • Remake Au:AA solutions over a different pH range
  • Calculate theoretical pH of Au:AA solutions
  • Prepare stock solutions for titration

Task List

Description

  • Theoretical pH Calculations

Mol of HAuCl4 (2.5 mM)

1 mL* 1 L/1000 mL * 2.5 mmol/1 L * 1 mol/1000 mmol = 2.5*10^-6 mol HAuCl4

Mol of NaOH (5 mM and 1 M stock)

0.5 mL * 1 L/1000 mL * 5 mmol/1 L * 1 mol/1000 mmol = 2.5*10^-6 mol NaOH
1 mL * 1 L/1000 mL * 5 mmol/1 L * 1 mol/1000 mmol = 5*10^-6 mol NaOH
0.1 mL * 1 L/1000 mL * 1 moll/1 L * 1 mol/1000 mmol = 1*10^-4 mol NaOH
1 mL * 1 L/1000 mL * 1 mol/1 L * 1 mol/1000 mmol = 1*10^-3 mol NaOH

Mol of NaOH - Mol of HAuCl4 = Mol of OH

1. 0
2. 2.5*10^-6 mol
3. 9.75*10^-5 mol
4. 9.975*10^-4 mol

Concentration = Mol NaOH/ 10mL * 1000mL/ 1 L

pH = -log(concentration)

Data

Amino Acid Solutions prepared last week did not work at any concentration.

Gold Nanoparticle and Nano fiber solutions prepared with BSA

Gold NanoParticles Sept.jpg

From left to right Au:BSA ratio: 60, 90, 120, 150

Nano particles made in 60, 90, and 120. Nano fibers made in 120 and 150. 90 darker than 60.