User:Meng Xiao He/Notebook/fall08/2008/11/30

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Gm/sucrose selection

  • all colonies were sucrose insensitive
  • all Duo colonies, some cbb colonies, and one cco colony was Gm sensitive
  • seems like sucrose selection isn't really working?
  • Gm- colonies picked for platinum colony pcr, with complex conditions (11-30)

new filter Duo

  • the 1 MR-1 colony that appeared on new cco filter plate was grown o/n in salt-free LB and streaked onto sucrose plate