User:Meng Xiao He/Notebook/fall08/2008/11/26

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Double checking cbb1, cbb12, Duo17, and Duo18 are all indeed Gm resistant WM cco is indeed DAP dependent and Gm resistant !! cbb1 and 12 seem to be sucrose resistant> entire streak had thick growth on sucrose plates Duo17 and 18 seem mostly sucrose sensitive> colonies thin out greatly by end of streak Updates Spin down Duo has 2 small MR-1 colonies cbb3 has many MR-1 colonies w/ diff sizes cco has 2 small MR-1 colonies 2 colonies from each plate streaked onto new LB Gm plate to ensure they're not contaminants Suicide vector loss Inoculated salt-free LB for new overnight cultures with Duo 17 and Duo 18 Conjugation cco w/ new filtration devices Colony PCR Platinum gradient PCR to test conditions for cbb and Duo Platinum PCR with 2 cco colonies at 56C and 1 direction determinant (relative to M13F sequencing primer)