User:Meng Xiao He/Notebook/fall08/2008/11/23

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Conjugation continued

  • Filter approach
    • filters vortexed (syringes and seals still attached) > LB DAP passed thru > LB Gm sucked thru filter into syringe > vortex again > fluid in syringe centrifuged > supernatant removed > wash with LB twice > cells plated on LB Gm and used to inoculate LB Gm liquid culture
    • filters wiped with EtOH externally, placed entirely within LB Gm to shake at 30°C
  • Centrifuge approach
    • cells pelleted > supernatant removed > washed with LB twice > streaked onto and spread onto LB Gm, used to inoculate LB Gm liquid culture