User:Meng Xiao He/Notebook/fall08/2008/11/14

Main project page Previous entry      Next entry

PCR results Top 2 lanes in ladders are 2kb and 800bp 1st 8: cco in pER21 candidate 2nd 8: cbb3 in pER21 candidate 3rd 8: Duo in TOPO C last 8: TOPO B, different colonies TOPO B3, B6, B7, and B10 seem correct Not quit sure what the other bands are, but the TOPO C stuff looks that size?? none of the conditions look particularly different, but this is a low res. gel temperature 7 seems like a good place to start alarmingly not clean TOPO C looks like it might only have 1/2 of what it's supposed to?