User:Meng Xiao He/Notebook/fall08/2008/11/13
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Ligated the Duo fragment from pUC19+Duo into new, old, and concentrated pER21 fragments
By PCR B2 is cbb3 & C1 is cco. The product was smeary, so not sure if they contain the full flank products, but they're not empty and not other things.
Ordered sequencing primers to look at KO plasmids. 2 for each flank; may not be sufficient for all cases to get entire read.