User:Meng Xiao He/Notebook/fall08/2008/11/11

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Sequencing results

M13R primer used

  • TOPO A is cco
  • TOPO B is empty
  • TOPO C is Duo
  • pUC19 + Duo does seem to be complete flank (alignment goes past middle site)
  • pER21 (taken from original glycerol stock strain) does NOT seem to contain the M13 reverse sequencing site

Transformation failure

  • Electroporated C1 and B2 DNA into wt MR-1> no colonies after 2 days on LB-GM selection