User:Meng Xiao He/Notebook/fall08/2008/11/06

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pir-116 maintains plasmids at high copy higher than glycerol stock strain, 300-400 ng/ul Distinguishing digests TOPO (3.5kb): EcoRI+PstI Duo: 1.2 kb, 300bp cco: 1.7 kb cbb3: 400, 830, 300 pER21+flanks (and flankless as ctrl): EcoRI + PstI>column>SmaI at 25 Gel 1: pER21+flanks, EP>SmaI digest 1-3: A1-3 100 bp ladder 5-7: B1-3 pER21 1kb ladder 10-12: C1-3 B2 is cbb3 candidate & C1 is cco candidate Gel 2 pER21: EP only 1kb ladder 3-5: TOPO A, B, C 100 bp ladder A1-3 EP only B1-3 EP only