User:Meng Xiao He/Notebook/fall08/2008/11/01

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PCR results Lanes 1-6: cbb3: 3, 5; cco: 3, 5; Duo: 3, 5 100bp ladder 8-10 Xover: cbb3, cco, Duo 1kb ladder X over product PCR Since X over products were not very concentrated and had other amplicons, set up 25ul PCRs of just those 3 using some purified product as the template. PCR conditions: MXFG program, column 5, extension time changed to 45s. This PCR ended up being very messy, with lots of smearing. There also appeared to be amplication of the fragments that made up the x-over individually. ?? XmaI o/n digest cbb, cco, Duo from Xover PCR (original) [45, 70, 60 ul of DNA digested+ 2 of enzyme and appropriate vol of NEB4] pUC19 pER21 old, concentrated old, and new vectors were 20ul DNA+1 of enzyme and necessary NEB4