User:Meng Xiao He/Notebook/fall08/2008/10/30

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PCR redo

10-30.mx.jpg

7-11 should be:

  • cbb3III 5
  • cbb3I 3
  • ccoIII 3
  • DuoII 5
  • DuoI 3

12???????

use columns 4, 6, 9, 11 on gradient mxfg, with dmso in 11 (.3ul)

clonewelled

Digest

BsmBI, overnight at 55, heat killed at 80 for 25min:

  • ccb3 (both)
  • cco (both)
  • Duo (both)

Rx:

  • 15 ul of each PCR clonewell frag
  • 3.55ul buffer 3
  • 2ul BsmBI