User:Meng Xiao He/Notebook/fall08/2008/10/28

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PCR

  • Previous flank PCR worked only for ccoII 5
  • Repeat with 30ul reactions and 57/55/57
    • cbb3III 5
    • cbb3I 3
    • ccoIII 3
    • DuoII 5
    • DuoI 3
  • 45s extension