User:Meng Xiao He/Notebook/fall08/2008/10/24

From OpenWetWare
< User:Meng Xiao He‎ | Notebook‎ | fall08‎ | 2008‎ | 10
Jump to: navigation, search
Report.pngMain project page
Next entryResultset next.png

<sitesearch>title=Search this page</sitesearch>

PCR flanking regions

Phusion:

  • cbb3III 5
  • cbb3I 3
  • ccoII 5
  • ccoIII 3
  • DuoII 5
  • DuoI 3

35 cycles:

  • 60°C annealing for 5 cycles, 58°C annealing for 30 cycles, 60°C for 5 cycles
  • 1kb extension time


Mix:

  • 2μL 25μM primer pair mix
  • 25μL 2x master mix
  • colony
  • 23μL H2O