- Synthesize Pepsin-AuNPs for reactivity tests tomorrow
- Determine the Au:Protein ratio required for fiber formation for pepsin and HRP
- Synthesis of HRP-AuNPs and Pepsin-AuNPs
- Into test tube that will contain a total of 5mL add
- Au solution such that the final concentration is 0.25mM
- Protein solution such that the final concentration reflects the following Au:Protein ratios
- Water up to 5mL
Source: Dr. Hartings
- UV-Vis taken: gold 1:10 dilution, HRP 1:2 dilution, Pepsin Stock, BSA-HRP 1:60 supernatant
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