User:Megan L. Channell/Notebook/Horseradish/2013/10/01

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Objective

To monitor the kinetics and yield of the HRP-catalyzed oxidation of luminol. These experiments will be compared to future experiments with HRP-functionalized nanoparticles.

From Dr. Hartings


Procedure

1. UV-Vis Absorbance of Reactants, Catalysts, and Products

  1. Take UV-Vis of horseradish peroxidase stock solution.
  2. Take UV-Vis of luminol of stock solution. The absorbance too high, so the stock solution was diluted 200x.
  3. Take UV-Vis of 3-aminophthalic acid.
  • Data not taken.

Figures

HRP-luminol-H2O2-UVVIS.jpg

Notes