User:Megan L. Channell/Notebook/Horseradish/2013/09/11
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Horseradish Peroxidase UV-Vis and Gold Solution
The protocol can be found from yesterdays lab. The only adjestment was that the diltuion of the HRP was a total of 10 mL instead of 2mL.
Determine how many atoms are in a AuNP
Use the calibration curve on group data, y=3×10-5x2+0.0209x
Solve for x
x= -703.51 and x=6.842
So, x= 6.842μg/mL
The concentration we used was diluted by a factor of 10. So actual concentration is .06842g/L
.006842g/L Au* (1 mol/ 196.96657 g Au)= 3.47x10-4 M of Au
The #Au Atoms/NP = [Au]/[citrate-AuNP] = (3.47x10-4 M)/( 9.17×10-9M)= 37,884 The concentration of citrate AuNP was found in a previous lab.
The amount of Au atoms in citrate-AuNP is 37,884