Objectives
- Finish polydopamine nanofiber scaffolds
- Wet-autoclave nanofiber coated scaffold in PBS
- Attempt to make silver:protein nanofibers
Sample Preparation: Polydopamine nanofibers
- Au stock solution:
- 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
- Myoglobin stock solution:
- 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
- Volumes of Au, protein, and water for each test tube (Myoglobin ratios from Febrary 11 pictured below):
- Final [Au]=0.5mM, 5mL total volume
Autoclaved Nanofiber-coated Scaffold
- Nanofiber coated scaffold from February 5 wet-autoclaved in PBS
- Though it appears the nanofibers remained on the scaffold, microscope images show that the scaffold was stained purple and very few nanofibers remained
- This may be due to autoclaving or to the length of time (14 days) the sample was immersed in PBS
Macro image:<br.>
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Micro images:<br.>
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Sample Preparation: Silver:BSA Nanofiber
- We will attempt to form silver:protein nanofibers in a similar method as gold:protein nanofibers, using silver (I) nitrate, BSA, and HCl to bring the pH up to 3 (according to Dr Fox's previous work)
- The final solution will have 0.25mM AgNO3, 1mM HCl, water, and defined ratios of BSA as detailed in the table below
- All samples will be heated to 80C in an oven for 4 hours
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