User:Mbennie/Notebook/Lab Notebook/Notebook/2007/08/01

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Cellular Adhesion

Signal Sequence (140 bp), Part 1 of IgA bc (527 bp), Part 2 of IgA bc (123 bp), Part 3 of IgA bc (236 bp), IgA bc full (892 bp), GCN4 Leucine Zipper (156 bp)
Signal Sequence (140 bp), Part 1 of IgA bc (527 bp), Part 2 of IgA bc (123 bp), Part 3 of IgA bc (236 bp), IgA bc full (892 bp), GCN4 Leucine Zipper (156 bp)
  • PCR
    • Performed PCR on N. gonorrhea and BBa_C2001
    • Tubes with 40ul PCR Supermix, .4ul N. gonorrhea DNA, and .4ul of each primer:
    • Tubes with 40ul PCR Supermix, 1ul cell dilution (picked colony diluted in 100ul water), and .4ul of each primer:
    • PCR protocol
      • 5mins@95C
      • 20secs@94C
      • 20secs@55C
      • 1min@68C
      • REPEAT 2-4, 34 times
      • 5mins@70C
      • FOREVER@4C
  • Gel
    • Ran 1% gel for 40 minutes at 100V with samples (5ul sample with 2ul of loading dye)
    • BANDS APPEAR TO BE THE RIGHT SIZE!!!
    • The colony PCR did not work as well as the others