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Transformation of standard components

  • Viewed plate from Thu, about a dozen colonies were flourescing brightly
  • Selected one colony for amplification:
  1. 5 mL LB medium, 50 μL of 5 mg/mL ampicillin, and one colony into a culture tube
  2. Shaken at 18 h at 37[[:Category:{{{1}}}|{{{1}}}]]