User:Matthew R Skorski/Notebook/471 - Exp BioChem/2016/03/29

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Objective

Today's objective is to make AuNP fibers using lysozyme and BSA.

Protocol

All of the fibers that I made yesterday formed AuNP. Today, we decided to decrease the amount of lysozyme in our samples in order to encourage better fiber formation. We changed the ratio of lysozyme:gold from 1:45 to 1:60.

We also decided to try making fibers using BSA because BSA might also encourage better fiber formation. The ratio of BSA:gold that we used was 1:120.

Additionally, we decided to make a batch of 9 lysozyme and 9 BSA samples to incubate in Rhodamine for 2 hours. We also decided to make a batch of 9 lysozyme and 9 BSA samples to incubate in Rhodamine for 24 hours. We made a batch of 7 lysozyme and 7 BSA samples ("blanks") that we will use to make a standard curve for the concentration of Rhodamine as a function of its UV-Vis absorbance.

The following reagents we used were as follows:

Screen Shot 2016-03-29 at 3.35.57 PM.png

We made the lysozyme samples as follows:

Screen Shot 2016-03-29 at 3.36.06 PM.png

We were going to make the BSA samples so that they would have a total volume of 10mL, but I screwed up and added too much water, so we made the samples so that they would have a total volume of 12.9mL. We made the BSA samples as follows:

Screen Shot 2016-03-29 at 3.42.22 PM.png

We then incubated the samples at 80 degrees Celsius for 4 hours.

Tomorrow, we will incubate samples for 2 and 24 hours. NOTE: We will have to adjust the volume of Rhodamine so that the final concentrations are 10, 5, and 2µM of Rhodamine in the samples for the BSA samples. I factored this in to our spreadsheet. We will also make both standard curves and then measure the absorbance of the 2 hour samples. We will measure the 24 hour samples on Thursday.