User:Matthew R Skorski/Notebook/471 - Exp BioChem/2016/03/23
Project name | Main project page Previous entry Next entry |
Objective
ProtocolMaking New AuNP FibersThe fibers that were synthesized yesterday all formed AuNP. Today, we will add HCl to our samples to increase the acidity. We've found that increasing acidity generally tends to increase fiber formation. The ingredients we used were as follows: We added the following to each sample: We did not label the samples because they were all the same. We will label the samples once we determine whether the synthesis worked. We then put the samples in the oven at 80 degrees Celsius for 4 hours (cycle P1). UV-Vis Absorbance: Standard CurveWe had been finding that measuring the absorbance of Rhodamine B dissolved in DI water was not yielding a linear calibration curve. After multiple trials, we found that measuring the absorbance of Rhodamine B dissolved in a solution of the ingredients used for the AuNP fiber sample gave a much more accurate calibration curve. We did the following to make our calibration curve:
We washed the cuvette out with DI water and then methanol between each run. UV-Vis Absorbance of Samples Synthesized on 20160316 and Incubated in Rhodamine for 24 HoursWe measured the absorbance of the samples that had been synthesized on March 16 and then incubated in 24 hours in Rhodamine B (from March 22 to March 23):
We washed the cuvette out with DI water and then methanol between each run. HPLCDr. Fox got the HPLC to work. We will use HPLC to measure acetylsalicylic acid and salicylic acid in solution. ResultsRhodamine B Standard CurveSee data for tomorrow UV-Vis Absorbance Measurements of Samples Synthesized on 20160316 and then Incubated in Rhodamine B for 24 HoursSee Michael's notebook
|