User:Matthew R Skorski/Notebook/471 - Exp BioChem/2015/11/03
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The objective of today was to run the Bradford Analysis of Protease Degradations for 1 nM Protinase K.
The protocol is identical to the one used on 9/29/15 except the fibers were spun down at 300 rpm for 10 minutes, not 1500 rpm for 1 minute.
The graph below shows the absorbance of the samples as function of the wavelength of incident light when corrected. The six samples were first corrected for by subtracting the absorbance of the superblank of buffer and Bradford reagents. Then the samples were corrected for again by subtracting the results of the first correction by the blanks for their corresponding time periods. Then, the isosbestic point at 535 nm from each samples was subtracted from all the wavelengths of that sample.
The graph below shows the peak for the absorbance at 600 nm. From 0 minutest to 75 minutes the aborbance rises, indicating more protein is free in solution. Then it drops at 135 minutes before rising again at 26 hour. More data points should be taken to ensure that the exact shape of the curve.