User:Mary Mendoza/Notebook/CHEM 581: Experimental Chemistry I/2014/11/14

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Bead Protocol III

  • Add 2 mL of PVA-clay into 10 mL of ethyl acetate. Stir at 300 rpm.
  • When PVA-clay holds its bead shape, add 200 uL of glutaraldehyde. Increase the speed to 320 rpm and only stir for 1 minute.
  • After 1 minute, slowly pour 7 mL of 0.2 M NaHCO3. Stir for 30 seconds.
  • Pour the mixture into a falcon tube and centrifuge for 1 min. at 3000 rpm.