User:Mary Mendoza/Notebook/CHEM 581: Experimental Chemistry I/2014/09/03

From OpenWetWare
Jump to navigationJump to search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

UV-Vis for Malachite Green Solutions

  • As stated in the protocol, the absorbance of the following malachite green solutions; 0ppm, 0.20ppm, 0.50ppm, 1.1ppm, 1.4ppm, 1.7ppm, and 2.0ppm Malachite Green, were taken.
  • The sample cell used was a quartz cuvette. The absorbance of each concentration was recorded from 200 to 800 nm.
  • Observing the absorbance of the malachite green solutions from the figure below, the two prominent maxima are at 423 and 617 nm.
Absorbance of Malachite Green Solutions
  • Dilution calculations for taking the absorbance of malachite green samples, 80 ppm MG in PVA and 200 ppm MG in PVA with clay, were performed by James Schwabacher and Jacob Esenther. Dilutions for these were performed to keep the absorbance under 1.
  • 200μL of 80 ppm MG from PVA film and 100μL of 200 ppm MG from PVA with clay film were diluted into, separate, 10 mL of distilled water.

Matt Hartings Even though you give attribution to who made these samples, you NEED to say how they were made.

  • Using the same quartz cuvette, absorbance of malachite green samples with the concentration of 2, 8, 80, and 200 ppm, were taken from 200 to 800 nm.
Absorbance of Malachite Green in Sample Films

Batch 3 Film Synthesis

  • 1.0256 g of PVA and 1.0272 g of PVA (make of Southern Clay Products) + 0.1176 g of sodium montmorillonite (make of MP Biomedicals) were dissolved in 7 mL of distilled water.
  • Casting of films were followed as stated in the original protocol.


  • Be wary of the wavelengths being recorded and saved on the data print table.