User:Mark X. Ling/Notebook/Micb 323/2009/02/10

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Experiment 5:

Part1: Gel Electrophoresis of Modified Restriction Enzyme

Part 2: Southern Transfer

2 10 pic.jpg

Part 3: Protein Assay(Bradford assay and BCA assay)

  • Bradford Assay Standard.
Tube # BSA (uL) H2O (uL)
1 0 100
2 10 90
3 15 85
4 20 80
5 25 75
6 30 70
7 35 65
8 40 60
  • Microwell Binichoninic ACid (BCA) Assay Standard.
BCA conc (ug/mL) BCA stock (uL) Water (uL)
2000 250 0
1502 188 60
1000 125 125
752 94 160
500 62.5 187.5
252 31.5 218.5
124 15.5 234.5
24 3 247

Post Lab comments

  • Big mistake, added SDS to lysate
  • did not do Bradford of recombinant
  • only did BCA but result was not good